>> bb/183085@153873
> The grain was shaken once a day
The grain will shake exactly once after pouring spore suspension, and then left alone and dark until the moment of complete fouling, do not do this again. The mycelium needs to form bonds, stretch its strands from grain to grain, sprout inwards, and you're his harak harak.
> Oats
I've never been good at it. Try selected wheat for germination from marketplaces or corn. Soak the clock for 12 in a gent solution and then cook for 40 minutes. Throw it on the coop, drain the water and fall asleep in the jar for 2/3. Then, according to the method of grandmother's twists: a jar in a high pan, throw a towel at the bottom, pour water a little above the grain level, make sure that it does not pop up. And you boil this farm on a slow fire for an hour and a half, then you cut the water and let the jar cool down right in the pan with the water. As it cooled to room temperature, you take out and immediately pour a suspension there.
Instead of aluminum tape on the covers, I advise you to try medium-thick foil. Cut off a large square, fold it in half, press it to the neck, smooth, bend the edges and hook the neck 2-3 tightly stretched rubber bands for money (1-2 burst during cooking, so always take more than one). With a syringe with spores, you pierce the foil right in the middle (don’t forget to lubricate everything with alcohol, burn the needle with a lighter and cool it also in alcohol), pour a couple of cubes without touching the grain, immediately remove the needle and glue the hole with a breathable patch of the Omnipore type (well, you can use a muddy disk). Shake it gently so that the slime inside spreads on the grain and puts it in the closet. If after two weeks there is no movement inside - the can for the ejection without opening, Trie Egain.
Another option is to try to buy new lids for spinning in a fixed price, drill a hole in the middle and boil a grain with lids by sealing a hole from water. Then you inoculate through it. I like foil more, the lids can rust after cooking.
> 2 ml of gentamicin
What a fucking gent pour, reduce, weigh a lot of it is not necessary, disputes are not too comfortable in it to swim. 0.5 ml per 10 ml syringe is enough, and the rest of the ampoule pour to the soaked grain.
Try to make a suspension on water for injection, the cleanest option. Or pull boiling water into the syringe and let it cool, then place part of the print in a new ziplock, pour water there, mumble while flushing the spores and pull back through the puncture in the side without opening the zip.
In short, divide the print and try different options, different grains, different cooking times, etc. Sooner or later it will. The print divided into 10 parts is 10 syringes of 5-10 ml, each at least 3 cans. Fill a bigger pot and make several cans shorter at once.